Expression and activities of SOD isozymes.

<p>Effect of curcumin on mRNA expression and enzymatic activity of SOD isozymes in liver of lymphoma bearing mice (A) RT-PCR of Mn-SOD, Cu/Zn-SOD and β-actin gene, (B) Densitometric scanning of Mn-SOD and Cu/Zn-SOD genes after normalization with β-actin, (C) Specific staining showing activity of SOD isozymes, (D) Densitometric scanning of the activity band of SOD isozymes. Livers of all six animals of each group was pooled separately and used for extraction of total RNA and proteins at non denaturing condition. Data represent mean ± S.E.M. #p<0.05 compared to N group and *p<0.05 compared to DL+DMSO group respectively. Cur is curcumin, M is 100 bp marker and bw is body weight. N, DL, DL+DMSO, DLT50, DLT100 and DLT150 represents normal, Dalton's lymphoma bearing, Dalton's lymphoma bearing mice treated with DMSO and Dalton's lymphoma bearing mice treated with 50, 100 and 150 mg curcumin/kg body weight dissolved in DMSO respectively.