VprBP enhances HIV-1 infection in MDDCs.

<p>(A) VprBP expression in MDDCs and monocytes was detected by Western blotting. (B–D) Effect of knocking down VprBP on HIV-1 infection of MDDCs. MDDCs were transfected with specific siRNA 1^# and 2^# or off-target siRNA, and knockdown of VprBP was confirmed by Western blotting at 48 h post-transfection (B); transfected MDDCs were infected by HIV-luc/VSV-G for an additional 5 days, and viral infection was detected by measuring luciferase activity; alternatively, (C) transfected cells were infected for an additional 5 days by wild-type or <i>vpr</i>-deficient replication-competent HIV-1/AD8, and viral replication was monitored by quantifying p24^gag in culture supernatant. Data are mean ± SD. Results are representative of three independent experiments. *<i>P</i><0.05 and ***<i>P</i><0.001, were considered significant difference in paired Student <i>t</i> test.