MIF/D-DT-dependent regulation of p53 and clonal proliferation are independent of AMPK.

<p>A: A549 cells were transfected with siRNA oligos as indicated for 72 h and cell lysates were analyzed by immunoblotting. B: A549 cells were transfected with siRNA oligos as indicated. After 48 h, 500 cells were plated in duplicate in 6 well plates. Colonies were stained with crystal violet and enumerated. Data shown are representative of two (B) or three (A) independent experiments.