Confocal analysis of P2×₇-R in the kidney at the 8^th week of protocol.

<p>a) Intracellular calcium concentration by confocal microscopy. Intensity was quantified using pseudocolor image according to fluorescence intensity by Fluo-4; these images showed the calcium mobilization in renal tissue when exposed to BzATP 100µM. Micrographies were obtained with x400 of magnification. b) ­Graphics of the calcium mobilization in relation to basal fluorescence. c) Quantification after the specific agonist. Each group with n = 5, Two-way ANOVA with Newman-Keuls post-test. p<0.05: a vs. CTL+SE; b vs. DM+SE. CTL+SE, sedentary control; CTL+SE+NAC, sedentary control plus NAC; CTL+EX, training control; CTL+EX+NAC, training control plus NAC; DM+SE, sedentary diabetic; DM+SE+NAC, sedentary diabetic plus NAC; DM+EX, training diabetic; DM+EX+NAC, training diabetic plus NAC; auf, arbitrary unit of fluorescence.