PD173074 inhibition of cell proliferation, cycle transition, and apoptosis resistance depends on the TC-1 expression level <i>in vitro</i>.

<p>(A) MTT assay. The optical density value was detected at a series of time points to evaluate cell proliferation. (B) Plate colony formation assay. The colonies were stained with crystal violet staining solution and counted, and the clone formation rate was then calculated. (C) Cell cycle assay. The percentage of cells in the S phase was measured using a flow cytometer, and the data were analyzed using the ModFit LT software. (D) Cell apoptosis assay. The cells were incubated in the dark in a solution containing PE-A and PerCP-Cy5.5. The percentage of apoptotic cells (lower right quadrant) were analyzed using a FACS equipped with a doublet discriminating module, and the data were analyzed using the CellQuest software. The columns represent the average colony formation rate (B), S-phase cell rate (C), and apoptosis cell rate (D) from at least three independent experiments. The bars show the SE. *indicates statistically significant changes (P<0.05) between the A549 group and the A549+PD173074 group.