AEG-1 regulates downstream substrates of Akt in HCC cells under suspension conditions.

<p>The AEG-1-over-expressing and AEG-1-knockdown cells were grown under suspension conditions. Cell lysates were prepared 24 h after suspension culture and immunoblotted with the indicated antibodies. GAPDH is shown as a loading control. Representative results are shown (left), and the mean +SD of the signal intensity of protein bands in three independent experiments is quantified (right; *<i>P</i><0.05 vs corresponding vector or siRNA control).