AEG-1 promotes orientation chemotaxis to HPMEC supernatant by HCC cells.

<p>(<b>A</b>) Control (Vector) and AEG-1-over-expressing SMMC-7221 cells (AEG-1-8 and AEG-1-12) were seeded onto plates that were pre-coated with 2% agar. After culture for 24 h, the suspension-cultured HCC cells were assessed for chemotaxis ability. The lower wells of a Boyden chamber were loaded with HPMEC supernatant or serum-free endothelial cell medium (ECM) as a control. Results from a representative experiment are shown (×400, HE). (<b>B</b> and <b>C</b>) MHCC-97H and HCC-LCM3 control (NC) and AEG-1 knockdown (siAEG-1-1 and siAEG-1-2) cells were assessed for chemotaxis ability as in (<b>A</b>). Results from representative experiments are shown (×400, HE). (<b>D</b>, <b>E</b> and <b>F</b>) Quantification (mean±SD) of five different fields of three independent experiments corresponding to the results in panels A-C are shown (*<i>P</i><0.05).