Internalized Aβ42 peptides were rapidly transported to lysosomes through an endolytic pathway.

<p>U87 cells were transfected with EGFP-Rab5 to mark early endosomes (a–c and j–l) or stained with LysoTracker Green to mark lysosomes (d–i and m–r). Cells were incubated with 0.4 µM sAβ42 (A) or oAβ42 (B) for various durations and live-cell images were taken by confocal microsopy. Scale bar is 20 µm. (C, D) Quantification of the Aβ42 peptides transport over time. The percentages of colocalization of Aβ42 peptides with EGFP-Rab5 (C) and LysoTracker Green (D) were analyzed and plotted. The error bars represent the average ± SEM of n>15 cells (C) and >25 cells (D).