Characterization of anti-Cthrc1 monoclonal antibodies.

<p>HEK293T cells (293) and CHO-K1 cells (CHO) were transduced with human or rat Cthrc1 expressing adenovirus. Cell lysates (CL) or conditioned media (CM) were collected for immunoblot using four different antibodies as indicated. The double band recognized by all the antibodies represents Cthrc1 with partially cleaved signal peptide as determined by mass-spectrometry (data not shown). Mouse monoclonal antibody 08G09 reacts with rat and mouse Cthrc1 but not human, rabbit monoclonal Vli-55 is specific for the conserved C terminus present in all species, mouse monoclonal 10G07 detects only the N terminal epitope of human Cthrc1, mouse monoclonal antibody 13E09 detects an internal epitope present in mouse, rat and human Cthrc1.