Phylogenetic and genomic analysis of the tarsier endogenous herpesvirus.

<p>Panel A: DNA polymerase tree showing the placement of the TsyrHVL sequence within the <i>Betaherpesvirinae</i>. Only the lineage leading to the node including TsyrHVL is shown and the rest are collapsed for clarity, and the size of the collapsed clade is arbitrary. Panel B represents the phylogeny reconstructed from a concatenated amino acid alignment of 6 core genes (terminase, large tegument, uracil-DNA glycosylase, kinase, capsid protein and helicase). Unclassified betaherpesviruses are shown as black branches, whereas those belonging to defined genera are indicated in colour. The rooting at <i>Proboscivirus</i> was determined according to the phylogeny in panel A. Numbers at each node in both Panel A and B represent bootstrap support. Panel C shows a schematic of the tarsier sequences mapped to HHV6 as a reference. Orange lines indicate wgs contigs obtained from NCBI and GenBank IDs are annotated. Contig ABRT02391417.1 is represented on both sides, since it consists entirely of the DR region, although it aligns with ABRT02259801.1 with only 5 differences, and both placements are plausible. Blue box indicates the virus' terminal direct repeat (DR) regions. Yellow boxes represent the major internal repeat regions. Because the genomes are so large, it is not feasible to represent the complete coding content. Instead major herpesvirus core blocks (HCB) are indicated (as in reference <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004332#pgen.1004332-Fields1" target="_blank">[5]</a>), and genes that are relevant to discussion points in the main text are also annotated. Abbreviations for Panel A and B are THEVE: Tarsier Herpesvirus Endogenous Viral Element, HHV6A/6B/7/5: <i>human herpesvirus 6A/6B/7/5</i>, MuHV1/2/8: <i>Murid herpesvirus 1/2/8</i>, AoHV1: <i>Aotine herpesvirus 1</i>, SaHV3: <i>Saimiriine herpesvirus 3</i>, PaHV2: <i>Panine herpesvirus 2</i>, CeHV5: <i>Cercopithecine herpesvirus 5</i>, TuHV1, PoCMV: <i>Porcine cytomegalovirus</i>.