Change of translation of individual mRNAs in LPS-activated macrophages.

<p>(A) Distribution of <i>Ncl</i>, <i>Nfkbiz</i> and <i>Cpd</i> mRNA across the four pools of polysome fractions as determined by microarray analysis (mean ± SD, n = 3). (B) The ribosome load is defined as the ratio (H/L) of the proportion of an mRNA in the heavy (H) and the light (L) pool. Mean H/L was determined for each detectable, coding and RefSeq-annotated mRNA by microarray analysis (n = 3). Highlighted in orange or blue are all mRNAs that show a shift from the general trend (orthogonal regression, solid black line) beyond the cut-off of two SD (dashed lines) in ≥2/3 biological replicates and the mean; R_P, Pearson's correlation coefficient. (C) The difference in ribosome load before and after LPS stimulation of RAW264.7 macrophages (Δ log₂(H/L)) was determined for 20 mRNAs by microarray analysis (n = 3) and qPCR (n = 4). (D) The difference in ribosome load before and after LPS stimulation (Δ log₂(H/L)) was determined for 19 mRNAs by qPCR in RAW264.7 cells (n = 4) and BMDM (n = 2).