Ppn1 and Ssu72 oppose condensin-mediated chromosome condensation.

<p><b>A.</b> Serial dilutions of the indicated strains were plated on rich media at the indicated temperatures. <b>B.</b> Chromosome segregation in anaphase was visualized after growing cells of the indicated genotypes for one generation at 34°C. Anaphases were scored as defective when lagging chromatin was detected between the two main DNA masses. For each genotype, a minimum of 6 independent experiments was performed in which a minimum of 100 anaphase cells was scored. <b>C.</b> The indicated strains were grown at 34°C for 3 hours and ChIP-qPCR was performed to analyze the amount of Cut14-GFP cross-linked to chromatin (mean ± standard deviation from 8 biological replicates). See text for details for the statistical analysis of the experiments. <b>D.</b> Tetrad dissection was used to show that the double mutant <i>ssu72Δ ppn1ΔPP1(ABC)</i> is dead. <b>E</b>. Serial dilutions of the indicated strains were plated on rich media at the indicated temperatures.