Testing for stress contribution to the <i>pak4</i> MO phenotype.

<p>(A) Injection of standard control MO (6 ng) into wild type and <i>pak4</i> mutant embryos. Live embryos at 3 dpf were anesthetized and photographed. Images shown are representatives of 92 out of 98 for wild type and 17 out of 17 for mutant. (B) Heat exposure of embryos. Wild type and F₃ mutants were exposed to 35°C from blastula stage to 24 hpf. Control samples (28.5°C) were incubated for 5 hours longer than the heated embryos in order to compensate for different developmental rates.