Real-time PCR quantification of <i>L. infantum</i> DNA in the liver of vaccinated vs non-vaccinated macaques.

<p>Parasite DNA was quantified in liver biopsy samples from vaccinated and control macaques at weeks 6 (upper panel) and 24 (lower panel) post-infection. Each point represents the value obtained for individual macaques. Lines represent mean value ± SD. Italic letters indicate significant differences (<i>p</i><0.01), when comparing the vaccinated groups submitted to either rA2 protein-plus-rhIL-12/alum, Ad5-A2/rA2+rhIL-12/alum, or DNA-A2/rAd5-A2 protocols to each of the control groups, <i>i.e</i>., PBS (<i>a</i>), rhIL-12/alum (<i>b</i>); G5: Ad5-ASP-2/alum (<i>c</i>), or DNA-wt/Ad5-ASP-2 (<i>d</i>).