Western blot analysis of membrane markers ZO-1, N-cadherin (N-CAD), Na-K ATPase (ATPase), connexin 43 (CX43), in cells isolated and expanded in DMEM + F12 medium supplemented with 10% heat-treated platelet releasate (HPR) or complete SHEM medium (FBS).

hDlg1 depleted cells show reduced levels of E-cadherin expression but increased levels of β-catenin expression.

Paola Massimi (152976)
Patrizia Zori (152978)
Sally Roberts (1688)
Lawrence Banks (152980)

July 2012

Cells were grown to 80% confluency and then extracted into cytosolic (F1), membrane (F2), nuclear...

Western blots of cell markers in fibrin-covered peritoneal mesothelial cells (PMCs).

Cheng-Chung Fang (135315)
Jenq-Wen Huang (135317)
Ren-Shi Shyu (135318)
Chung-Jen Yen (135320)
Cheng-Hsiang Shiao (135322)
Chih-Kang Chiang (135324)
Rey-Heng Hu (135326)
Tun-Jun Tsai (135328)

September 2012

Equal amounts of protein (20 µg per lane) from untreated or fibrin-covered PMCs were resolved, tr...

Protein N-myristoylation of SAMM50 but not of TOMM40 is required for binding of protein to the membrane.

Toshihiko Utsumi (297331)
Kanako Matsuzaki (787872)
Aya Kiwado (5963435)
Ayane Tanikawa (5963438)
Yuki Kikkawa (5963441)
Takuro Hosokawa (5963444)
Aoi Otsuka (5963447)
Yoshihito Iuchi (3630712)
Hirotsugu Kobuchi (297321)
Koko Moriya (297322)

November 2018

Total cell extracts of non-transfected COS-1 cells [A] and COS-1 cells transfected with cDNA encodin...

Western blot of whole cell (WC) homogenates and apical membrane fractions from two immortalized hRPTC lines grown in Transwell membranes.

John J. Gildea (845025)
Peng Xu (17962)
Brandon A. Kemp (5089898)
Julia M. Carlson (5089901)
Hanh T. Tran (5089883)
Dora Bigler Wang (5089889)
Christophe J. Langouët-Astrié (5089895)
Helen E. McGrath (5089892)
Robert M. Carey (5089886)
Pedro A. Jose (191382)
Robin A. Felder (845024)

April 2018

Arrows on the left-hand side of the blots indicate the molecular sizes, while the arrows on the r...

Photomicrographs of immunostaining showing expression of the membrane markers ZO-1 A), Na+K+/ATPase (B), connexin 43 (C), and N-cadherin (D) immunostaining of a confluent layer of corneal endothelial cells (CECs) isolated and expanded in DMEM + F12 medium supplemented with 10% heat-treated platelet releasate (HPR).

Ming-Li Chou (580583)
Thierry Burnouf (580584)
Tsung-Jen Wang (580585)

June 2014

(Cell nuclear markers were stained with DAPI. Proteins were clearly localized at plasma membranes...

Western blot analysis.

Amol K. Bhandage (6083420)
Zhe Jin (43692)
Sergiy V. Korol (425541)
Atieh S. Tafreshiha (6083423)
Priya Gohel (4907518)
Charlotte Hellgren (790278)
Daniel Espes (2628670)
Per-Ola Carlsson (302173)
Inger Sundström-Poromaa (752726)
Bryndis Birnir (113570)

December 2018

Western blot analysis showing the expression of Ca2+ sensor protein stromal interaction molecules (S...

Lectin blots of total membranes and immunopurified Kv3.1 and E-cadherin proteins from transfected CHO cell lines.

M. Kristen Hall (455173)
Douglas A. Weidner (455174)
Jian ming Chen (455175)
Christopher J. Bernetski (455176)
Ruth A. Schwalbe (352646)

September 2013

Total membranes (∼25 µg) from Pro-5, Lec1, and LEC10B cells transfected with wild type...

Expression of cytodifferentiation markers in PC-3M-1E8 and PC-3M-2B4 cells.

Shujiang Zhang (5917007)
Chengcheng Zheng (5917010)
Shunheng Yao (5917013)
Zhonghui Wang (4089880)
Li Xu (108702)
Rongfu Yang (5917016)
Xiang Meng (383515)
Jianhui Wu (385334)
Li Zhou (54356)
Zuyue Sun (5917019)

October 2018

Western blot analysis was performed on whole cell lysate using antibodies against epithelial (CK8), ...

Western blot analysis of membrane protein extracts isolated from instantaneous transfected and nontransfected HEK293 cells.

Jian Wang (5901)
Yongxin She (767086)
Miao Wang (248377)
Maojun Jin (767084)
Yongfei Li (801715)
Jing Wang (4865)
Yuan Liu (88411)

September 2015

Equal amounts of proteins were loaded for each of the strains, separated by gel electrophoresis, ...

Western blot analysis in adherent and spheroid cells.

Carlo Mischiati (700287)
Blendi Ura (700288)
Leda Roncoroni (700289)
Luca Elli (700290)
Carlo Cervellati (700291)
Monica Squerzanti (700292)
Dario Conte (700293)
Luisa Doneda (700294)
Patrizia Polverino de Laureto (110842)
Giorgia de Franceschi (5662270)
Roberta Calza (700295)
Carlos A. Barrero (433330)
Salim Merali (433334)
Carlo Ferrari (179895)
Carlo M. Bergamini (700296)
Enzo Agostinelli (660855)

March 2015

The figure presents a representative experiment obtained with SK-ChA-1. Ad: adherent; Sp: spheroi...

Validation of the plasma membrane (PM) purification.

Catalina Petrareanu (448413)
Alina Macovei (175469)
Izabela Sokolowska (448414)
Alisa G. Woods (448415)
Catalin Lazar (175466)
Gabriel L. Radu (448416)
Costel C. Darie (448417)
Norica Branza-Nichita (175475)

August 2013

Equal amounts of proteins from total HepaRG cell lysate (TL) and purified PM fraction were loaded...

Characterization of the cell fraction used for channel incorporation.

Sajjad Salari (736104)
Maedeh Ghasemi (736105)
Javad Fahanik-Babaei (736106)
Reza Saghiri (736107)
Remy Sauve (736108)
Afsaneh Eliassi (736109)

May 2015

Membranes were probed with organelle specific antibodies. A) Plasma membrane marker: Actin (C-11)...

Western blotting analysis of membrane subfractions isolated from Synechocystis.

Tiago Toscano Selão (585925)
Lifang Zhang (130786)
Candan Ariöz (585926)
Åke Wieslander (585927)
Birgitta Norling (585928)

February 2014

Each gel lane was loaded with 5 µg membrane protein and probed with the antibodies indicated. PM1...

Immunofluorescent staining and quantification of ID-related proteins of the EHTs cultured for 3, 7 and 14 days.

Jin Zhou (90734)
Yao Shu (485612)
Shuang-Hong Lü (485613)
Jun-Jie Li (309727)
Hong-Yu Sun (2607592)
Rong-Yu Tang (485614)
Cui-Mi Duan (485615)
Yan Wang (15435)
Qiu-Xia Lin (485616)
Yong-Chao Mou (485617)
Xia Li (14984)
Chang-Yong Wang (485618)

November 2013

(A–D) At day 3, the cell membranes were positively stained with N-cadherin, Cx43, PKP2 and...

Biochemical characterization of membrane- and nucleus-associated PrPc isoforms.

Etienne Morel (35915)
Stéphane Fouquet (85773)
Carine Strup-Perrot (85774)
Cathy Pichol Thievend (85775)
Constance Petit (85776)
Damarys Loew (85777)
Anne-Marie Faussat (60168)
Lucile Yvernault (85778)
Martine Pinçon-Raymond (85779)
Jean Chambaz (85780)
Monique Rousset (85781)
Sophie Thenet (85782)
Caroline Clair (85783)

February 2013

(A): Stability of membrane and nuclear PrPc was analyzed by western blot after treatme...

hDlg1 depleted cells show reduced levels of E-cadherin expression but increased levels of β-catenin expression.

Paola Massimi (152976)
Patrizia Zori (152978)
Sally Roberts (1688)
Lawrence Banks (152980)

July 2012

Cells were grown to 80% confluency and then extracted into cytosolic (F1), membrane (F2), nuclear...

Western blots of cell markers in fibrin-covered peritoneal mesothelial cells (PMCs).

Cheng-Chung Fang (135315)
Jenq-Wen Huang (135317)
Ren-Shi Shyu (135318)
Chung-Jen Yen (135320)
Cheng-Hsiang Shiao (135322)
Chih-Kang Chiang (135324)
Rey-Heng Hu (135326)
Tun-Jun Tsai (135328)

September 2012

Equal amounts of protein (20 µg per lane) from untreated or fibrin-covered PMCs were resolved, tr...

Protein N-myristoylation of SAMM50 but not of TOMM40 is required for binding of protein to the membrane.

Toshihiko Utsumi (297331)
Kanako Matsuzaki (787872)
Aya Kiwado (5963435)
Ayane Tanikawa (5963438)
Yuki Kikkawa (5963441)
Takuro Hosokawa (5963444)
Aoi Otsuka (5963447)
Yoshihito Iuchi (3630712)
Hirotsugu Kobuchi (297321)
Koko Moriya (297322)

November 2018

Total cell extracts of non-transfected COS-1 cells [A] and COS-1 cells transfected with cDNA encodin...

Western blot of whole cell (WC) homogenates and apical membrane fractions from two immortalized hRPTC lines grown in Transwell membranes.

John J. Gildea (845025)
Peng Xu (17962)
Brandon A. Kemp (5089898)
Julia M. Carlson (5089901)
Hanh T. Tran (5089883)
Dora Bigler Wang (5089889)
Christophe J. Langouët-Astrié (5089895)
Helen E. McGrath (5089892)
Robert M. Carey (5089886)
Pedro A. Jose (191382)
Robin A. Felder (845024)

April 2018

Arrows on the left-hand side of the blots indicate the molecular sizes, while the arrows on the r...

Photomicrographs of immunostaining showing expression of the membrane markers ZO-1 A), Na+K+/ATPase (B), connexin 43 (C), and N-cadherin (D) immunostaining of a confluent layer of corneal endothelial cells (CECs) isolated and expanded in DMEM + F12 medium supplemented with 10% heat-treated platelet releasate (HPR).

Ming-Li Chou (580583)
Thierry Burnouf (580584)
Tsung-Jen Wang (580585)

June 2014

(Cell nuclear markers were stained with DAPI. Proteins were clearly localized at plasma membranes...

Western blot analysis.

Amol K. Bhandage (6083420)
Zhe Jin (43692)
Sergiy V. Korol (425541)
Atieh S. Tafreshiha (6083423)
Priya Gohel (4907518)
Charlotte Hellgren (790278)
Daniel Espes (2628670)
Per-Ola Carlsson (302173)
Inger Sundström-Poromaa (752726)
Bryndis Birnir (113570)

December 2018

Western blot analysis showing the expression of Ca2+ sensor protein stromal interaction molecules (S...

Lectin blots of total membranes and immunopurified Kv3.1 and E-cadherin proteins from transfected CHO cell lines.

M. Kristen Hall (455173)
Douglas A. Weidner (455174)
Jian ming Chen (455175)
Christopher J. Bernetski (455176)
Ruth A. Schwalbe (352646)

September 2013

Total membranes (∼25 µg) from Pro-5, Lec1, and LEC10B cells transfected with wild type...

Expression of cytodifferentiation markers in PC-3M-1E8 and PC-3M-2B4 cells.

Shujiang Zhang (5917007)
Chengcheng Zheng (5917010)
Shunheng Yao (5917013)
Zhonghui Wang (4089880)
Li Xu (108702)
Rongfu Yang (5917016)
Xiang Meng (383515)
Jianhui Wu (385334)
Li Zhou (54356)
Zuyue Sun (5917019)

October 2018

Western blot analysis was performed on whole cell lysate using antibodies against epithelial (CK8), ...

Western blot analysis of membrane protein extracts isolated from instantaneous transfected and nontransfected HEK293 cells.

Jian Wang (5901)
Yongxin She (767086)
Miao Wang (248377)
Maojun Jin (767084)
Yongfei Li (801715)
Jing Wang (4865)
Yuan Liu (88411)

September 2015

Equal amounts of proteins were loaded for each of the strains, separated by gel electrophoresis, ...

Western blot analysis in adherent and spheroid cells.

Carlo Mischiati (700287)
Blendi Ura (700288)
Leda Roncoroni (700289)
Luca Elli (700290)
Carlo Cervellati (700291)
Monica Squerzanti (700292)
Dario Conte (700293)
Luisa Doneda (700294)
Patrizia Polverino de Laureto (110842)
Giorgia de Franceschi (5662270)
Roberta Calza (700295)
Carlos A. Barrero (433330)
Salim Merali (433334)
Carlo Ferrari (179895)
Carlo M. Bergamini (700296)
Enzo Agostinelli (660855)

March 2015

The figure presents a representative experiment obtained with SK-ChA-1. Ad: adherent; Sp: spheroi...

Validation of the plasma membrane (PM) purification.

Catalina Petrareanu (448413)
Alina Macovei (175469)
Izabela Sokolowska (448414)
Alisa G. Woods (448415)
Catalin Lazar (175466)
Gabriel L. Radu (448416)
Costel C. Darie (448417)
Norica Branza-Nichita (175475)

August 2013

Equal amounts of proteins from total HepaRG cell lysate (TL) and purified PM fraction were loaded...

Characterization of the cell fraction used for channel incorporation.

Sajjad Salari (736104)
Maedeh Ghasemi (736105)
Javad Fahanik-Babaei (736106)
Reza Saghiri (736107)
Remy Sauve (736108)
Afsaneh Eliassi (736109)

May 2015

Membranes were probed with organelle specific antibodies. A) Plasma membrane marker: Actin (C-11)...

Western blotting analysis of membrane subfractions isolated from Synechocystis.

Tiago Toscano Selão (585925)
Lifang Zhang (130786)
Candan Ariöz (585926)
Åke Wieslander (585927)
Birgitta Norling (585928)

February 2014

Each gel lane was loaded with 5 µg membrane protein and probed with the antibodies indicated. PM1...

Immunofluorescent staining and quantification of ID-related proteins of the EHTs cultured for 3, 7 and 14 days.

Jin Zhou (90734)
Yao Shu (485612)
Shuang-Hong Lü (485613)
Jun-Jie Li (309727)
Hong-Yu Sun (2607592)
Rong-Yu Tang (485614)
Cui-Mi Duan (485615)
Yan Wang (15435)
Qiu-Xia Lin (485616)
Yong-Chao Mou (485617)
Xia Li (14984)
Chang-Yong Wang (485618)

November 2013

(A–D) At day 3, the cell membranes were positively stained with N-cadherin, Cx43, PKP2 and...

Biochemical characterization of membrane- and nucleus-associated PrPc isoforms.

Etienne Morel (35915)
Stéphane Fouquet (85773)
Carine Strup-Perrot (85774)
Cathy Pichol Thievend (85775)
Constance Petit (85776)
Damarys Loew (85777)
Anne-Marie Faussat (60168)
Lucile Yvernault (85778)
Martine Pinçon-Raymond (85779)
Jean Chambaz (85780)
Monique Rousset (85781)
Sophie Thenet (85782)
Caroline Clair (85783)

February 2013

(A): Stability of membrane and nuclear PrPc was analyzed by western blot after treatme...

hDlg1 depleted cells show reduced levels of E-cadherin expression but increased levels of β-catenin expression.

Paola Massimi (152976)
Patrizia Zori (152978)
Sally Roberts (1688)
Lawrence Banks (152980)

July 2012

Cells were grown to 80% confluency and then extracted into cytosolic (F1), membrane (F2), nuclear...

Western blots of cell markers in fibrin-covered peritoneal mesothelial cells (PMCs).

Cheng-Chung Fang (135315)
Jenq-Wen Huang (135317)
Ren-Shi Shyu (135318)
Chung-Jen Yen (135320)
Cheng-Hsiang Shiao (135322)
Chih-Kang Chiang (135324)
Rey-Heng Hu (135326)
Tun-Jun Tsai (135328)

September 2012

Equal amounts of protein (20 µg per lane) from untreated or fibrin-covered PMCs were resolved, tr...

Protein N-myristoylation of SAMM50 but not of TOMM40 is required for binding of protein to the membrane.

Toshihiko Utsumi (297331)
Kanako Matsuzaki (787872)
Aya Kiwado (5963435)
Ayane Tanikawa (5963438)
Yuki Kikkawa (5963441)
Takuro Hosokawa (5963444)
Aoi Otsuka (5963447)
Yoshihito Iuchi (3630712)
Hirotsugu Kobuchi (297321)
Koko Moriya (297322)

November 2018

Total cell extracts of non-transfected COS-1 cells [A] and COS-1 cells transfected with cDNA encodin...

Western blot analysis of membrane markers ZO-1, N-cadherin (N-CAD), Na-K ATPase (ATPase), connexin 43 (CX43), in cells isolated and expanded in DMEM + F12 medium supplemented with 10% heat-treated platelet releasate (HPR) or complete SHEM medium (FBS).

Abstract

Extracted data

Western blot analysis of membrane markers ZO-1, N-cadherin (N-CAD), Na-K ATPase (ATPase), connexin 43 (CX43), in cells isolated and expanded in DMEM + F12 medium supplemented with 10% heat-treated platelet releasate (HPR) or complete SHEM medium (FBS).

Abstract

Extracted data

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