The composition of colon DCs and macrophages is altered in inflamed Muc2^−/− mice.

<p>LP cells from the colon of Muc2^−/− mice, inflamed Muc2^−/− mice and Muc2^+/− controls were stained for DCs and macrophages and analyzed by flow cytometry. (<b>A</b>) The gating strategy used is shown where viable MHC-II⁺NK1.1⁻TCR⁻CD19⁻ cells (left column) were distinguished by CD11c expression (middle) and CD11c⁺MHC-II⁺ cells were then analyzed for CD103 and CD11b (right). The numbers are the percent of cells in the indicated gates. (<b>B–D</b>) The frequency of CD103⁺CD11b⁻ DCs (<b>B</b>), CD103⁺CD11b⁺ DCs (<b>C</b>), and CD103⁻ macrophages (<b>D</b>) among MHCII⁺CD11c^hi cells in the indicated gates is shown. (<b>E–G</b>) The absolute number of CD103⁺CD11b⁻ (<b>E</b>), CD103⁺CD11b⁺ (<b>F</b>) and CD103⁻ macrophages (<b>G</b>) among 10⁶ viable LP cells is shown. Each symbol represents an individual mouse. The median is indicated by a horizontal line. Data are from 15 independent experiments that analyzed 20–29 mice per group. The mice were between 8–19 weeks of age. Statistical significance between groups was assessed using the Kruskal-Wallis test followed by Dunn’s multiple comparison test. Significance is indicated as *p<0.05, **p<0.01, while all other comparisons are non-significant.