hOMSC developed neuronal-like morphology and gene expression after DA differentiation protocol.

<p>Bright field microscopy of hOMSC cultured in serum free medium for 17 days and of hOMSC-DA (A–B), scale bar 200 µM. Real time PCR analysis of differentiated hOMSC-DA from four different donors (C), showing a reduction in the expression of Nanog, Oct4 and Sox2 and a concomitant increased expression of the neuronal markers β-III tubulin and NCAM1 compared to the naïve hOMSC which served as baseline (relative value = 1). Differentiated hOMSC also showed increased expression of genes related to dopaminergic differentiation and mature dopaminergic neurons. Data is expressed as Mean ± SEM. Significance levels: *p<0.05, **p<0.01, ***p<0.001.