Subset-specific LPS-induced gene signatures.

<p>(A–B) Dendritic cells were stimulated in vivo with an intraperitoneal injection of 25ug LPS and their activation confirmed by measuring upregulation of CD86 24 hours later. (C–E) LPS stimulated DC subsets isolated from a second cohort of mice (n = 10, spleen cells pooled) were magnetic bead enriched, sorted, analysed by RNA-Seq, and then compared to steady-state controls (n = 5, spleen cells pooled). (C) Differences in LPS-induced gene expression were visualised in a Venn diagram. (D–E) Heatmaps showing the expression of key immune effector genes (D) uniquely regulated in either CD8 (left) or CD11b DCs (right) or (E) similarly regulated in both subsets. Data are presented as fold changes (+LPS/−LPS).