Postoperative evaluations in rabbit models.

<p>The rabbits were divided into four treatment groups: ‘Control group’ is the filtration surgery only group (a). Group injected with 0.1 ml 5% w/v PTMC₁₅-F127-PTMC₁₅/MMC (0.1 mg/ml) hydrogel in filtration surgery (b). Treat with 0.5 mg/ml MMC for 5 minutes during filtration surgery group (c). Group with 0.1 ml 5% w/v PTMC₁₅-F127-PTMC₁₅ injected in filtration surgery (d). (A) Survival curve of filtering blebs after glaucoma filtration surgery using Kaplan-Meier analysis. Filtering blebs in group ‘a’ (n = 4) and ‘d’ (n = 4) mostly failed within 1 weeks. Group ‘b’ (n = 4) markedly increased the bleb survival period. In group ‘c’ (n = 4), the bleb survived nearly 4 weeks. Kaplan-Meier analysis showed a significant difference in the survival distributions among the four groups. (Log Rank = 75.121, p<0.01). (B) IOP changes within postoperatively 28 days. The results were obtained and expressed as the mean± SD. There is no significant difference between each group (p>0.05). (C) Proliferating cell nuclear antigen (PCNA) was analyzed in each group at post-operative day 28.(D) Apoptotic cells were indicated by TUNEL assay in each group at day 28.