Mapping of mouse cervical lymph nodes by high frequency ultrasound.

<p><b>A.</b> Overview image of the HF US platform for cervical lymph node evaluation. An anesthetized B6 mouse is shown positioned on the Vevo 2100 heated imaging platform with the ventral side exposed. Each paw is tapped to a monitoring electrode and the rectal probe (blue) secured to the stage. The transducer (white) is positioned over the ventral neck area. <b>B.</b> Diagram showing relative locations of murine cervical lymph nodes. Individual neck sections visualized by HF US imaging and histology are indicated by dashed lines. Arrows denote specific positions of each mapped section relative to corresponding ultrasound and histology images. Each imaged anatomical location is numbered. M, mandibular node. AM, accessory mandibular node. SP, superficial parotid node. <b>C.</b> Serial transverse sections of the mouse neck imaged by HF US corresponding to the indicated anatomic regions in (B). <b>D.</b> Transverse cervical H&E stained histological sections corresponding to the HF US sections in (C). Arrows labeled “2” denote mandibular node as diagrammed in B. Scale bar = 1 mm. CP, cheek pouch. VT, ventral tongue. DT, dorsal tongue. E, esophagus.