<p>(A) The paired-gRNA target sites of <i>Emx1</i> gene found by sgRNAcas9 program and the paired-gRNA searching mode. (B) Illustration of one pair sgRNA targeting at exon1 of <i>Emx1</i>.</p
Summary: Cas9 nucleases can be programmed with single guide RNAs (sgRNAs) to mediate gene editing. H...
(A) Schematic depiction of the GR66 locus and sgRNA targeting sites. The sgRNA targeting sites, name...
<p>Sequence of sgRNAs and primers used for CRISPR/Cas9 knockout cell generation and evaluation.</p
<p>(A) Searching off-target sites for each sgRNA targeting at human <i>Emx1</i> (hEMX1) gene. (B) Cl...
<div><p>Genome engineering has been revolutionized by the discovery of clustered regularly interspac...
<p>(A) Schematic illustrating the target sequence (blue) and corresponding PAM (red). PCR amplificat...
<p><b>A.</b> Target sequences for CRISPR-Cas9-derived nucleases are composed of two components, the ...
<p>(<b>A</b>) Schematic illustration of the CRISPR/Cas system. The guide RNA (gRNA) binds to the 20-...
Targeting specificity is an essential issue in the development of CRISPR-Cas technology. Using a luc...
Although the CRISPR/Cas9/sgRNA system efficiently cleaves intracellular DNA at desired target sites,...
<p>(<b>A</b>) Overview of the AdV Cas9 and gRNA constructs used in this study. CRISPR/Cas9 AdV const...
Genome engineering has been revolutionized by the discovery of clustered regularly inter-spaced pali...
<p>Individual components in both panels are not drawn to scale. <b>A)</b> RNA guided nuclease Cas9 s...
Although the CRISPR/Cas9/sgRNA system efficiently cleaves intracellular DNA at desired target sites,...
(A) Schematic representation of the sgRNA sequence targeted in Exon I of Sfabd-A locus. (B) Differen...
Summary: Cas9 nucleases can be programmed with single guide RNAs (sgRNAs) to mediate gene editing. H...
(A) Schematic depiction of the GR66 locus and sgRNA targeting sites. The sgRNA targeting sites, name...
<p>Sequence of sgRNAs and primers used for CRISPR/Cas9 knockout cell generation and evaluation.</p
<p>(A) Searching off-target sites for each sgRNA targeting at human <i>Emx1</i> (hEMX1) gene. (B) Cl...
<div><p>Genome engineering has been revolutionized by the discovery of clustered regularly interspac...
<p>(A) Schematic illustrating the target sequence (blue) and corresponding PAM (red). PCR amplificat...
<p><b>A.</b> Target sequences for CRISPR-Cas9-derived nucleases are composed of two components, the ...
<p>(<b>A</b>) Schematic illustration of the CRISPR/Cas system. The guide RNA (gRNA) binds to the 20-...
Targeting specificity is an essential issue in the development of CRISPR-Cas technology. Using a luc...
Although the CRISPR/Cas9/sgRNA system efficiently cleaves intracellular DNA at desired target sites,...
<p>(<b>A</b>) Overview of the AdV Cas9 and gRNA constructs used in this study. CRISPR/Cas9 AdV const...
Genome engineering has been revolutionized by the discovery of clustered regularly inter-spaced pali...
<p>Individual components in both panels are not drawn to scale. <b>A)</b> RNA guided nuclease Cas9 s...
Although the CRISPR/Cas9/sgRNA system efficiently cleaves intracellular DNA at desired target sites,...
(A) Schematic representation of the sgRNA sequence targeted in Exon I of Sfabd-A locus. (B) Differen...
Summary: Cas9 nucleases can be programmed with single guide RNAs (sgRNAs) to mediate gene editing. H...
(A) Schematic depiction of the GR66 locus and sgRNA targeting sites. The sgRNA targeting sites, name...
<p>Sequence of sgRNAs and primers used for CRISPR/Cas9 knockout cell generation and evaluation.</p
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