Mitochondrial function analysis of <i>ob/ob</i> and lean livers following different durations of cold ischaemia (hours).

<p>A: <i>Ob/ob</i> livers demonstrated lower Complex I (CI) oxidative phosphorylation throughout cold ischaemia and were significantly lower from lean livers at earlier time points. B: CI+Complex II (CII) oxidative phosphorylation did not differ between groups. C: After 8 hours of cold ischaemia, <i>ob/ob</i> and lean livers showed significantly lower CI respiration to CII respiration ratio compared to 3 hours of cold ischaemia. <i>Ob/ob</i> livers also showed significantly lower CI/CII ratios compared to lean livers after 16 hours of cold ischaemia. Data are shown as mean ± SEM (n = 9 mice/group; <i>ob/ob</i>, closed circle; lean livers, open circle). Restricted maximum likelihood with post-hoc Tukey-Kramer analysis was performed. *, <i>P</i>&lt;0.05 (vs. lean); #, <i>P</i>&lt;0.05 (vs. 3 hours).