Detection of IFN-γ and TNF in lung tissue.

<p>(<b>A</b>) Intracellular IFN-γ was analysed in lung mononuclear cells from influenza A virus infected animals and control animals by flow cytometry. IFN-γ ⁺ cells were gated among live lymphocytes. Plots show representative isotype control (left) and IFN-γ staining (right) from the same infected animal on day 3 pi (<b>B</b>) Percentages of IFN-γ⁺ cells obtained by flow cytometry in control animals (<i>n</i> = 8) and infected animals (<i>n</i> = 4 per day). Data from one animal at day 1 is missing due to too few cells isolated. (<b>C</b>) Gene expression for IFN-γ and TNF mRNA in infected animals (<i>n</i> = 4 per day) was calculated as relative values to the household gene GADPH and to mRNA levels of the target gene in a control group (<i>n</i> = 4). Each symbol represents one infected animal, different symbols represents animals sacrificed the same day. Values above 1 indicate an up regulation, whereas values below 1 indicate a down regulation of the target gene.