Clustergram of antibody gene repertoires.

<p>The frequency of each V_H(D)J_H recombination was determined for each of nine tissues, and a clustergram was created. V_H(D)J_H recombinants were clustered by relative frequency in each tissue-specific repertoire, and the resulting phylogenetic tree is shown on the left. Tissue-specific repertoires were clustered by the overall V_H(D)J_H usage of each repertoire, and the resulting clustering diagram is shown at the top. The frequency variation for each V_H(D)J_H recombination across all tissue-specific repertoires was determined, and standardized to a range of −3 to 3. A complete list of the frequency variation of all V_H(D)J_H recombinants for each tissue-specific repertoire, along with statistical significance and false discovery rate (FDR) calculations, is available in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0100839#pone.0100839.s002" target="_blank">File S1</a> (see eight files, each named Suppl Info_PB_vs_<i>tissue</i>).