Effects of heparin binding on E-E₃₄₅K and E-E₃₂₇G mutants infectivity and recombinant DENV-4 E-DIII proteins.

<p>Results from heparin binding assays of DENV-4 E-E₃₄₅K, DENV-4 E-E₃₂₇G, and DENV-4 viruses, determined by virus removal using (A) heparin-sepharose beads and (B) hyaluronic acid-EAH sepharose 4B beads. Recombinant DENV-4 wild type and mutants E-DIII proteins were expressed from E. coli (C) and the purified proteins and stained with Coomassie blue. (D) heparin binding ELISA was used to determine E-DIII proteins-heparin binding activity. (* indicates statistical significance at <i>p</i><0.05; n.s. indicates none statistical significance).