CD4+ T-cell phenotypes in peripheral blood of adults before and after antiretroviral therapy at 3, 6 and 12 months ART and in healthy HIV-uninfected individuals.

<p>(<b>A</b>) expansion of CD4 T- cell counts (0mth, n = 43; 3mths, n = 43; 6mths, n = 41; 12mths, n = 34; HIV-, 23) (<b>B</b>) naive CD4+ T cells (T_N: CD45RA+CCR7+), central memory CD4+ T cells (T_CM: CD45RA-CCR7+) and effector memory CD4+ T cells (T_EM: CD45RA-CCR7-) (0mth, n = 35; 3mths, n = 34; 6mths, n = 34; 12mths, n = 26; HIV-, 29) (<b>C</b>) CD28-CD57+ senescent cells (0mth, n = 34; 3mths, n = 34; 6mths, n = 39; 12mths, n = 20; HIV-, 10) (<b>D</b>) CD4+CD25^hiFoxP3+ T regulatory cells (0mth, n = 33; 3mths, n = 30; 6mths, n = 30; 12mths, n = 22; HIV-,13) Black horizontal bars represent median and IQRs. Wilcoxon matched pairs were used to compare T-cell characteristics of HIV-infected persons on ART over time, and the Mann Whitney U test in the HIV-infected and HIV-uninfected comparisons. Representative flow cytometric data demonstrating gating strategy are shown in <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0100640#pone.0100640.s001" target="_blank">Figure S1</a></b>.