Effect of MK-0626 on the expression of 8-hydroxy-2′-deoxyguanosine, manganese superoxide dismutase and heme oxygenase-1 during tacrolimus-induced pancreatic islet injury.

<p>(A and B) Immunohistochemistry for 8-hydroxy-2′-deoxyguanosine (8-OHdG) and its quantitative analysis in islet show intense nuclear expression and larger positive area for 8-OHdG in tacrolimus (TAC) groups. These changes are markedly decreased in TAC plus MK-0626 (M)-treated groups. (C) The TAC-induced serum 8-OHdG level is lowered by the addition of MK-0626. Immunoblot analysis of manganese superoxide dismutase (MnSOD) (D) and heme oxygenase-1 (HO-1, inducible form) and HO-2 (constitutive form) (E) in isolated pancreatic islets from the experimental rats. Note that the level of MnSOD and HO-1/HO-2 is lower in the TAC group; however, MK-0626 treatment leads to the recovery of its expression. Original magnifications, x400. <i>n</i> = 8 rats per group. ^#P<0.05 vs. control. ^$P<0.05 vs. TAC.