Schematic representation of the antigen selection process.

<p>Download selection is represented: first, 50 <i>P. vivax</i> antigens containing α-helical coiled coil motifs, selected from orthologues of <i>P. falciparum</i>, were chemically synthesized and tested with PNG sera. Seventeen reactive with prevalence >30% were tested with Colombian sera. Eight antigens with prevalence >50% either with PNG or Colombian sera were used for mice immunization and immune response testing. Four antigens were finally selected for further pre-clinical testing.