Ischemic insults affect the protein levels of the AMPAR subunits GluA1 and GluA2.

<p>(<b>A</b>) Total protein extracts were prepared 7 h and 24 h after the OGD insult and Western blot analysis were performed. Total GluA1 is decreased after 24 h, whereas GluA2 levels remain unaltered following both periods of recovery. The left panel shows a representative Western blot for total GluA1 and GluA2 present in cell lysates after OGD. The right panel represents the quantification of the Western blots. Bars represent the mean ± SEM of five independent experiments, performed in ditinct preparations. (<b>B</b>) GluA1 and GluA2 surface levels were analyzed after biotinylation of cultured hippocampal neurons, 24 h after the OGD insult. At this time point after OGD GluA1 is removed from the surface, whereas GluA2 surface levels remain unaltered. The left panel shows a representative Western blot for surface GluA1 and GluA2 after the insult. Total actin (from the total extract) was used as loading control. Bars represent the mean ± SEM of 3–4 independent experiments, performed in distinct preparations. *<i>p</i><0.05, as determined by the Student's <i>t</i>-test.