Sister chromatid cohesion loss occurs despite retention of cohesin enrichment along chromosome arm and pericentromeric CAR sites.

<p>(A) Position of primers used in ChIP along individual arm (comprising two CAR sites) and pericentromeric CAR sites for chromosome III. (B) Mcd1 enrichment along chromosome arm CARs are averages of three independent experiments obtained using four oligo pairs (34, 35, 36 and 37) in wildtype and <i>pds5-1</i> mutant cells. (C) Immunoprecipitation efficiency obtained using Quantitative PCR performed on CAR sites 36 and 37 confirm the Mcd1 enrichment levels observed using ChIP in both wildtype and <i>pds5-1</i> mutant cells. (D) Mcd1 enrichment along pericentromeric CARs obtained from nine oligo pairs (70, 72, 74, 76, 48, 78, 80, 82 and 84) in wildtype and <i>pds5-1</i> mutant cells. All primer design and designations from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0100470#pone.0100470-Glynn1" target="_blank">[5]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0100470#pone.0100470-Unal3" target="_blank">[72]</a>.