Agonism of GPBAR1 on human monocytes modulates a subset of LPS activated genes and blocks M1 differentiation.

<p>Monocytes from 5 donors were primed with IFNγ and stimulated with LPS ± agonist. RNA was isolated from these samples and subjected to RNA-Seq. (A) Heatmap of genes whose activation or repression by LPS at 6 hours was inhibited by the GPBAR1 agonist EX00000246. (B) Cellular function data from Ingenuity Systems was mapped onto the GPBAR1 regulated genes. (C) Heatmap of genes annotated as cytokine or chemokine whose activation or repression by LPS at 6 hours, 24 hours, or both times was inhibited by the GPBAR1 agonist EX00000246. (D) Individual plots of <i>Ccr7</i>, <i>Il12b</i> expression and ratio of <i>Il12/Il10</i> expression after stimulation with LPS treated with EX00000246.