LPS increases Cx43 expression.

<p>HPAECs were treated with LPS (200 ng/mL) for 6 hr or 24 hr and collected for Western Blot or qRT-PCR analyses. A.) A representative Western blot is shown. B.) Cx43 protein expression was normalized to β-actin and expressed relative to control. **, p<0.001. n = 6 for control, n = 3 for LPS (6 hr), n = 7 for LPS (24 hr). C.) Real-time RT-PCR analysis was performed on cells treated with LPS (24 hr) or control cultures. Cx43 mRNA levels were normalized to GAPDH mRNA. *, p<0.05, n = 14. C = control.