Flow cytometric analysis of gp120 expression on the surface of HIV-transfected HEK293T cells, in the absence or presence of chloroquine.

<p>Two days after transfection in the absence or presence of chloroquine to produce WT, N260Q or N260Q/S128N virus, HEK293T cells were stained to detect gp120 on the cell surface with the primary antibody 2G12 and a secondary anti-human antibody labelled with Alexa Fluor 647. Data are the means (± SEM) of 5 independent experiments.