Ingested human IGF1 increased JNK phosphorylation in <i>A. stephensi</i> midguts.

<p>Mosquitoes were fed blood meals containing 0.013 µM or 0.133 µM IGF1 or an equivalent volume of PBS. Midguts were dissected at 0.5–1 h after the initiation of blood feeding. Data are represented as the average fold induction±SEM of phospho-JNK quantified by densitometry and normalized first to the GAPDH loading control and then to the buffer control. A representative western blot is shown below the graph. Data were analyzed by Mann Whitney test. Experiments were replicated four times and p-values are displayed on the graphs.