<p>(A) Western blots of cell lysates with antibodies to collagen 3-hydroxylation complex components. Lysates are derived from two independent cultures for each genotype. (B) SDS-Urea PAGE analysis of steady-state labeled type I collagen from wild-type (+/+), heterozygous (+/−) and homozygous (−/−) fibroblasts (FB) and osteoblasts (OB). (C) Differential scanning calorimetry (DSC) analysis reveals no differences in thermal stability (T<sub>m</sub>) of type I collagen secreted by fibroblast cultures.</p
A method to simultaneously quantify the production, secretion, and prolyl hydroxylation of individua...
<p>Synthesis of collagen I and III was measured by capture sandwich ELISA (for collagen I expression...
<p>Cells were isolated from newborn wild type and <i>Col6a1</i> null mice and cultured for 4 days. (...
<p>Quantitation of type I collagen modifications at α1(I) and α2(I) K87 residues, which are importan...
Matched human skin fibroblast cultures were established from papillary and reticular dermis. Papilla...
<p>(a) Representative WB analysis of type II and type I collagen after 21 days in static or dynamic ...
<p>A). Procollagen processing and total amounts of soluble collagen recovered from primary fibroblas...
<p>A, DSC denaturation thermograms of pepsin-purified collagen (solid lines) and procollagen (dotted...
<p>(A) <i>Left</i>, Deposition of type I collagen by osteoblasts into extracellular matrix in cultur...
<p>As markers of differentiation we analyzed the expression of bone-related genes, BSP (A), ALP (B) ...
Three-dimensional lattices of reconstituted, polymerized type I collagen were subjected to partial h...
Fibrillar collagen type 1 is the most abundant type of collagen within the body and is a critical co...
ABSTRACT: Two cloned cell lines were isolated from cultures of mouse bone-marrow cells. One of the l...
Cultured skin fibroblasts from seven consecutive cases of lethal perinatal osteogenesis imperfecta (...
<p><b>A.</b> Immunofluorescence analysis of Type I Collagen deposition on wild-type and β1<sup>Ost-k...
A method to simultaneously quantify the production, secretion, and prolyl hydroxylation of individua...
<p>Synthesis of collagen I and III was measured by capture sandwich ELISA (for collagen I expression...
<p>Cells were isolated from newborn wild type and <i>Col6a1</i> null mice and cultured for 4 days. (...
<p>Quantitation of type I collagen modifications at α1(I) and α2(I) K87 residues, which are importan...
Matched human skin fibroblast cultures were established from papillary and reticular dermis. Papilla...
<p>(a) Representative WB analysis of type II and type I collagen after 21 days in static or dynamic ...
<p>A). Procollagen processing and total amounts of soluble collagen recovered from primary fibroblas...
<p>A, DSC denaturation thermograms of pepsin-purified collagen (solid lines) and procollagen (dotted...
<p>(A) <i>Left</i>, Deposition of type I collagen by osteoblasts into extracellular matrix in cultur...
<p>As markers of differentiation we analyzed the expression of bone-related genes, BSP (A), ALP (B) ...
Three-dimensional lattices of reconstituted, polymerized type I collagen were subjected to partial h...
Fibrillar collagen type 1 is the most abundant type of collagen within the body and is a critical co...
ABSTRACT: Two cloned cell lines were isolated from cultures of mouse bone-marrow cells. One of the l...
Cultured skin fibroblasts from seven consecutive cases of lethal perinatal osteogenesis imperfecta (...
<p><b>A.</b> Immunofluorescence analysis of Type I Collagen deposition on wild-type and β1<sup>Ost-k...
A method to simultaneously quantify the production, secretion, and prolyl hydroxylation of individua...
<p>Synthesis of collagen I and III was measured by capture sandwich ELISA (for collagen I expression...
<p>Cells were isolated from newborn wild type and <i>Col6a1</i> null mice and cultured for 4 days. (...