Immunofluorescence microscopy of a mantle frozen tissue section with anti-nephrin antibodies.

<p>(<b>A</b>) Cluster of rhogocytes at 250x primary magnification, showing positive immune reaction with guinea pig anti-nephrin antibodies. Note negative reaction of the mantle epithelium (arrow). (<b>A’</b>) The same section as in (A), labeled with the DNA stain DAPI (diamidino-2-phenylindole) to visualize the cell nuclei (arrow, mantle epithelium). (<b>B–B”</b>) The boxed area in (A, A’) at 1000x primary magnification, shown in phase contrast optics (B), epifluorescence optics with anti-nephrin antibodies (B’), and epifluorescence optics with DAPI stain (B”). Two rhogocytes are highlighted in boxes. Note that the positive reaction is mostly restricted to the cell periphery.