Expression and Functional Characterization of the <i>Agrobacterium</i> VirB2 Amino Acid Substitution Variants in T-pilus Biogenesis, Virulence, and Transient Transformation Efficiency

<div><p><i>Agrobacterium tumefaciens</i> is a phytopathogenic bacterium that causes crown gall disease by transferring transferred DNA (T-DNA) into the plant genome. The translocation process is mediated by the type IV secretion system (T4SS) consisting of the VirD4 coupling protein and 11 VirB proteins (VirB1 to VirB11). All VirB proteins are required for the production of T-pilus, which consists of processed VirB2 (T-pilin) and VirB5 as major and minor subunits, respectively. VirB2 is an essential component of T4SS, but the roles of VirB2 and the assembled T-pilus in <i>Agrobacterium</i> virulence and the T-DNA transfer process remain unknown. Here, we generated 34 VirB2 amino acid substitution variants to study the functions of VirB2 involved in VirB2 stability, extracellular VirB2/T-pilus production and virulence of <i>A. tumefaciens</i>. From the capacity for extracellular VirB2 production (ExB2⁺ or ExB2⁻) and tumorigenesis on tomato stems (Vir⁺ or Vir⁻), the mutants could be classified into three groups: ExB2⁻/Vir⁻, ExB2⁻/Vir⁺, and ExB2⁺/Vir⁺. We also confirmed by electron microscopy that five ExB2⁻/Vir⁺ mutants exhibited a wild-type level of virulence with their deficiency in T-pilus formation. Interestingly, although the five T-pilus⁻/Vir⁺ uncoupling mutants retained a wild-type level of tumorigenesis efficiency on tomato stems and/or potato tuber discs, their transient transformation efficiency in <i>Arabidopsis</i> seedlings was highly attenuated. In conclusion, we have provided evidence for a role of T-pilus in <i>Agrobacterium</i> transformation process and have identified the domains and amino acid residues critical for VirB2 stability, T-pilus biogenesis, tumorigenesis, and transient transformation efficiency.</p>