Effects of morroniside on the expression of Ang-1 and its receptor Tie-2 in the peri-infarct cortex of rats 7 days after cerebral ischemia.

<p>(A) Immunohistochemical staining of vessel-associated Tie-2 in the peri-infarct region (arrows), and new vessels with incomplete Ang-1-positive endothelial-like cell lining 7 days after ischemic/reperfusion treatment. Scale bar, 100 µm, and 50 µm for the enlarged image of Tie-2. (B) Double immunofluorecence staining of vWF with Ang-1 and Tie-2 indicated Ang-1 and Tie-2 were localized in endothelial cells. Scale bar, 50 µm. (C) Protein expression of Ang-1 (60 kDa) and Tie-2 (140 kDa) in the ipsilateral cortex 7 days after MCAO by immunoblotting. Quantitative analysis of Ang-1 and Tie-2 is expressed as a fraction of the respective levels of β-actin. n = 3 for each group. The values are the mean ± SEM. ^#<i>p<</i>0.05 and ^##<i>p<</i>0.01 versus sham-operated control group; **<i>p<</i>0.01 and ***<i>p<</i>0.001 versus vehicle-treated ischemic group.