BCL6 protein is weakly expressed in peripheral pre-cDCs during in vivo development.

<p>BM, peripheral blood, spleens and LNs were collected from naïve C57BL/6 mice. <i>(A)</i> BM HSCs and CDPs were gated as indicated. The Lin markers include CD3, CD4, CD8, CD19, B220 and CD11b. Representative FACS data were shown (n = 8). <i>(B)</i> Pre-cDCs were gated as indicated usingthe Lin markers including CD3, CD19 and B220. Representative FACS plots were shown (n = 6). Mouse IgG1 isotype control (solid grey), BCL6 expression in indicated DC population (open black). BCL6 expression gates were drawn based on the negative expression of isotype controls. Numbers in the FACS plots indicated the percent of population. <i>(C)</i> Overlay of BCL6 expression levels in different cell population. BCL6 expression in GC B cells obtained 7 days p.i. (gated as B220⁺ Fas⁺ CD38^lo cells) served as positive controls (open grey line) and naïve B cells (gated as B220⁺ Fas⁻ CD38^hi cells) as negative controls (open grey dashed line).