Complex 5 inhibits NF-κB transactivation activity.

<p>(A) RAW264.7 cells pretreated with or without complex <b>5</b> for 4 h were treated with LPS for 4 h. The expression of TNFα, IFNβ, IL6 and IP10 were measured by QPCR. (B) HeLa S3 cells pretreated with or without complex <b>5</b> for 4 h were stimulated with TNFα for 4 h. The expression of TNFα, IL8, CCL5, ICAM1, IL6 and A20 were measured by QPCR. (C) K562 or KCL22 cells pretreated with or without 50 µg/ml of complex <b>5</b> for 4 h were stimulated with 100 ng/ml TNFα for 2 h. The expression of IL8 and TNF were measured by QPCR. (D) 293T-luc reporter cells pretreated with or without various concentrations of complex <b>5</b> were stimulated with TNFα for 12 h. Cell lysates were prepared and the luciferase activities were measured. Error bars represent the variation range of duplicate experiments. Asterisks represents statistical significance by Student's <i>t</i>-test (*P<0.05).