Response of the Hepatic Transcriptome to Aflatoxin B₁ in Domestic Turkey (<i>Meleagris gallopavo</i>)

<div><p>Dietary exposure to aflatoxin B₁ (AFB₁) is detrimental to avian health and leads to major economic losses for the poultry industry. AFB₁ is especially hepatotoxic in domestic turkeys (<i>Meleagris gallopavo</i>), since these birds are unable to detoxify AFB₁ by glutathione-conjugation. The impacts of AFB₁ on the turkey hepatic transcriptome and the potential protection from pretreatment with a <i>Lactobacillus</i>-based probiotic mixture were investigated through RNA-sequencing. Animals were divided into four treatment groups and RNA was subsequently recovered from liver samples. Four pooled RNA-seq libraries were sequenced to produce over 322 M reads totaling 13.8 Gb of sequence. Approximately 170,000 predicted transcripts were <i>de novo</i> assembled, of which 803 had significant differential expression in at least one pair-wise comparison between treatment groups. Functional analysis linked many of the transcripts significantly affected by AFB₁ exposure to cancer, apoptosis, the cell cycle or lipid regulation. Most notable were transcripts from the genes encoding E3 ubiquitin-protein ligase Mdm2, osteopontin, S-adenosylmethionine synthase isoform type-2, and lipoprotein lipase. Expression was modulated by the probiotics, but treatment did not completely mitigate the effects of AFB₁. Genes identified through transcriptome analysis provide candidates for further study of AFB₁ toxicity and targets for efforts to improve the health of domestic turkeys exposed to AFB₁.</p>