Kinetic analysis of FWCS.

<p>(<b>A</b>) The sensorgram shows concentrations of FWCS passed over the immobilized p38α on the Ni-NTA chip for the calculation of binding constants. (<b>B</b>) The line diagram of IC₅₀ obtained by <i>in vitro</i> kinase assay after incubating the peptide with p38α for 30 mins, 2 h and 3 h with ten different concentrations of FWCS respectively using ATF-2 as a substrate. Error bar shows mean±SD.