Vitamin C attenuated LPS-induced NF-κB translocation.

<p>(A) Gel images of the western blot analyses of the p65 protein extracted in cytoplasmic and nuclear fractions from the control cultures (no treatment) or cultures treated with Vit. C (10 mM) alone, LPS (100 ng/ml) alone, or combined Vit. C-LPS for 60 min. PARP (a nuclear loading control) and β-actin (a cytoplasmic loading control) were used as internal controls to ensure equal protein loading. (B) Quantitative comparison of the relative p65 levels in cytoplasmic and nuclear fractions extracted from cultures that underwent various treatments. The data are represented as the mean ± SEM based on 3 experiments. ** <i>p</i><.01 and *** <i>p</i><.001 versus Cont.; ⁺<i>p</i><.05, ⁺⁺⁺<i>p</i><.001 versus LPS alone.