Trim28 deficiency alters the expression of EMT markers.

<p><i>A</i>, control and Trim28 knockdown A549 cells were stained (as described under “Experimental Procedures”) and examined using confocal immunofluorescence microscopy, as follows: E-cadherin (green, top panel), β-tubulin (green, bottom panel), and DAPI (blue). Merged images are in the bottom of each panel. <i>B</i>, RNA extracts of control and Trim28 knockdown A549 and H358 cells were subjected to real-time PCR to determine the expression of target genes as indicated. <i>Asterisks</i> represent significant <i>p</i> values, *: <i>p</i><0.05. <i>C</i>, the expression of EMT markers E-cadherin and N-cadherin were measured in control and Trim28 knockdown A549 and H358 cells by western blotting.