Recognition of distinct Aβ species by sera from mice immunized with differently adjuvanted formulations of the LPS-free (1–11)E2 antigen.

<p><b>panel a</b> Representative 1 µm×1 µm atomic force microscopy images of the indicated Aβ42 species (see ‘<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0101474#s2" target="_blank">Materials and methods</a>’ for details). <b>panel b</b> Immuno-dot blot analysis conducted on 60 ng each of the various Aβ42 species (<i>M</i>, monomers; <i>O</i>, oligomers; <i>P</i>, protofibrils; <i>F</i>, fibrils) at a 1∶5000 dilution of pooled (day 77) sera from B6C3/F1 mice immunized with Alhydrogel- or Addavax-adjuvanted LPS-free (1–11)E2, or with the same antigen without adjuvant (PBS) as indicated. <b>panel c</b> Immuno-dot blot analysis conducted on 15 ng each of the various Aβ42 species at a 1∶5000 dilution of pooled (day 77) sera from B6C3/F1 mice immunized with Alhydrogel- or Addavax-adjuvanted LPS-free (1–11)E2, or with the same antigen without adjuvant (PBS).