uPAR is required for MMS-induced PSMD6 phosphorylation.

<p>A. SiCo and uPARsi human VSMC were treated with 1.2(A) and serine phosphorylation (B) was assessed using Duolink proximity ligation assay. The right panels show the number of Duolink PLA signals per cell quantified using ImageJ software. DraQ5 was used as nuclear stain. C. Serine phosphorylation of PSMD6 was assessed by immunoprecipitation. PSMD6 from lysates of control and MMS-stimulated cells was immunoprecipitated. P-Ser was detected by western blotting. The lower panel shows loading controls.