Opposite modulation of cardiomyocyte cholinergic machinery by isoproterenol and pyridostigmine.
- Mariana Gavioli (592360)
- Aline Lara (154106)
- Pedro W. M. Almeida (592361)
- Augusto Martins Lima (592362)
- Denis D. Damasceno (592363)
- Cibele Rocha-Resende (592364)
- Marina Ladeira (592365)
- Rodrigo R. Resende (592366)
- Patricia M. Martinelli (5659807)
- Marcos Barrouin Melo (592367)
- Patricia C. Brum (145324)
- Marco Antonio Peliky Fontes (592368)
- Robson A. Souza Santos (592369)
- Marco A. M. Prado (154150)
- Silvia Guatimosim (154143)
Publication date
July 2014
DOIAbstract
<p><b>A–C</b>. qPCR experiments show that cardiomyocytes from ISO-rats present upregulation of VAChT, ChAT and M<sub>2</sub> muscarinic receptor mRNA levels. Cholinesterase inhibition prevented these effects. Lower levels of VAChT, ChAT and M<sub>2</sub> ACh muscarinic receptor transcripts were observed in cardiomyocytes from pyridostigmine treated rats. <b>D.</b> Downregulation of β1-AR transcript is observed in ventricular myocytes from ISO-rats, while pyridostigmine-treated rats presented opposite results. Cholinesterase therapy partially reversed the effect of ISO on β1-AR transcripts. n = 4–6 cardiomyocyte preparations from each group. *p<0.05 when compared to control group and #p<0.05 when compared to the ISO-treated rat.</p
Extracted data
Topics
cardiomyocytesAnatomical structure
muscarinic receptorProtein
pyridostigmineChemical substance
cardiomyocyteAnatomical structure
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ISOAgent
CholinesteraseBiomolecule
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