<p>(A) R3A-(+), -(−), or -Udel-infected MDMs, or uninfected control MDMs, were co-cultured with autologous CD4<sup>+</sup> T cells for 6 h. As a control, 5 µM NVP was added to parallel MDM-T cell co-cultures. All T cells were separated from the MDMs, and aliquoted for qPCR and western blot analyses. For qPCR analysis, T cells were lysed immediately, and Gag DNA levels quantified by qPCR and normalised to GAPDH. The levels of contaminating MDM-derived Gag and GAPDH DNA were subtracted from the total DNA levels. Graphs for the “no NVP” samples show the means ± SD of triplicate samples from a representative experiment relative to R3A-(+) (set at 100%). Single values are shown for the “5 µM NVP” samples. For western blot analyses, T cells were ...
<p>(A) IFN-β-treated MDMs were incubated for 20 min on ice with 10 µg/ml monoclonal Tetherin antibod...
AbstractTetherin is a host cell restriction factor that acts against HIV-1 and other enveloped virus...
<p>MDDCs were either untreated or exposed to HIV-1<sub>BaL</sub>(pellet), HIV-1<sub>BaL</sub>(CD45<s...
<p>(A) Schematic outline of the assay used to quantify cell-cell transmission from MDMs to autologou...
<p>(A) MDMs were infected with R3A-(+), -(−), or -Udel for seven days, lysed, and analysed by wester...
<p>(A) MDMs were infected with HIV-1 BaL for seven days, co-cultured with autologous CD4<sup>+</sup>...
<p>Jurkat cells were infected with the indicated HIV-1 mutant at an MOI of 1. 16 h later the cells w...
<p>(<b>A</b>) A barrier was placed into the cell-free path of HIV at the step of virus release by ex...
<p>Target CD4<sup>+</sup> T cells (1×10<sup>6</sup>) were treated for 16 h with H<sub>2</sub>O<sub>2...
<p>(A) R3A-infected MDMs were incubated for 1 h on ice in media containing 10 µg/ml polyclonal Tethe...
The IFN-inducible antiviral protein tetherin (or BST-2/CD317/HM1.24) impairs release of mature HIV-1...
<div><p>Bst-2/Tetherin inhibits the release of HIV by tethering newly formed virus particles to the ...
<p>A. Virus particle release was tested in 293T cells transfected with HIV-1ΔVpuΔNef NL4.3 proviral ...
Abstract Background Tetherin (BST-2/CD317/HM1.24) is an interferon (IFN)-inducible factor of the inn...
Background. Dendritic cells and their subsets, located at mucosal surfaces, are among the first imm...
<p>(A) IFN-β-treated MDMs were incubated for 20 min on ice with 10 µg/ml monoclonal Tetherin antibod...
AbstractTetherin is a host cell restriction factor that acts against HIV-1 and other enveloped virus...
<p>MDDCs were either untreated or exposed to HIV-1<sub>BaL</sub>(pellet), HIV-1<sub>BaL</sub>(CD45<s...
<p>(A) Schematic outline of the assay used to quantify cell-cell transmission from MDMs to autologou...
<p>(A) MDMs were infected with R3A-(+), -(−), or -Udel for seven days, lysed, and analysed by wester...
<p>(A) MDMs were infected with HIV-1 BaL for seven days, co-cultured with autologous CD4<sup>+</sup>...
<p>Jurkat cells were infected with the indicated HIV-1 mutant at an MOI of 1. 16 h later the cells w...
<p>(<b>A</b>) A barrier was placed into the cell-free path of HIV at the step of virus release by ex...
<p>Target CD4<sup>+</sup> T cells (1×10<sup>6</sup>) were treated for 16 h with H<sub>2</sub>O<sub>2...
<p>(A) R3A-infected MDMs were incubated for 1 h on ice in media containing 10 µg/ml polyclonal Tethe...
The IFN-inducible antiviral protein tetherin (or BST-2/CD317/HM1.24) impairs release of mature HIV-1...
<div><p>Bst-2/Tetherin inhibits the release of HIV by tethering newly formed virus particles to the ...
<p>A. Virus particle release was tested in 293T cells transfected with HIV-1ΔVpuΔNef NL4.3 proviral ...
Abstract Background Tetherin (BST-2/CD317/HM1.24) is an interferon (IFN)-inducible factor of the inn...
Background. Dendritic cells and their subsets, located at mucosal surfaces, are among the first imm...
<p>(A) IFN-β-treated MDMs were incubated for 20 min on ice with 10 µg/ml monoclonal Tetherin antibod...
AbstractTetherin is a host cell restriction factor that acts against HIV-1 and other enveloped virus...
<p>MDDCs were either untreated or exposed to HIV-1<sub>BaL</sub>(pellet), HIV-1<sub>BaL</sub>(CD45<s...