Dose-dependent effect of cytokines on interendothelial junction protein expression in HBMvECs.

<p>Confluent cells were treated with TNF-α (<b>A</b>) or IL-6 (<b>B</b>) (0–100 ng/ml, 18 hrs). Post-treatment, whole cell protein lysates were harvested for Western blotting. Histograms represent the densitometric fold change in relative protein expression for VE-cadherin, occludin and claudin-5 (bars reading left to right) in response to increasing concentration of cytokine. *<i>P</i>≤0.05 versus untreated control. All gels are representative.