Apoptosis inhibition of macrophages by MPT64 protein.

<p>PMA-differentiated RAW264.7 cells were put into 24-well flat bottom tissue culture plates at a density of 1×10⁵ cells per well. Then, RAW264.7 macrophages were incubated with PBS, BCG-PPD (10 µg/ml) or a mixture of PPD-MPT64 at a different concentrations for 16 h. (A) Apoptosis was detected by measuring the membrane exposure of PS using annexin V by flow cytometry, and the results were analyzed. <b>Fig. 2A</b> Significant differences of the apoptosis percentages. *<i>P</i><0.05, the PPD group vs the MPT64 (10 µg/ml) group; ** <i>P</i><0.01, the PBS group vs the PPD group, and the PPD group vs the MPT64 (15 µg/ml or 20 µg/ml) group. The apoptosis percentage is not significantly different for the GST group or heat-treated MPT64 protein compared with the PPD group. <b>Fig. 2A</b> Ne, the PBS group; HAMPT64, heat treated MPT64; M10, MPT64 at 10 µg/ml of concentration; M15, MPT64 at 15 µg/ml of concentration; M20, MPT64 at 20 µg/ml of concentration. <b>Fig. 2B</b> A: The PBS group. B: The PPD group. C: The MPT64 (15 µg/ml) group. Data shown are representative of three independent experiments.